[分享]Microfluidic的应用领域

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[color=#0000FF]Microfluidic devices can be used to obtain a variety of interesting measurements including molecular diffusion coefficients [1,2], fluid viscosity [3], pH [4,5], chemical binding coefficients [1] and enzyme reaction kinetics [6-8}. Other applications for microfluidic devices include capillary electrophoresis [9], isoelectric focusing [5,10,11], immunoassays [12-15], flow cytometry [16], sample injection of proteins for analysis via mass spectrometry [17-19], PCR amplification [20-22], DNA analysis [23-26], cell manipulation [27], cell separation [28], cell patterning [29,30] and chemical gradient formation [31,32]. Many of these applications have utility for clinical diagnostics [33,34].

1.Kamholz, A. E., Weigl, B. H., Finlayson, B. A. & Yager, P. Quantitative analysis of molecular interaction in a microfluidic channel: The T-sensor. Analytical Chemistry 71, 5340-5347 (1999).
2.Kamholz, A. E., Schilling, E. A. & Yager, P. Optical measurement of transverse molecular diffusion in a microchannel. Biophysical Journal 80, 1967-1972 (2001).
3.Galambos, P. Ph.D. Thesis, Mechanical Engineering. University of Washington, Seattle (1998).
4.Weigl, B. H. & Yager, P. Silicon-microfabricated diffusion-based optical chemical sensor. Sensors and Actuators B-Chemical 39, 452-457 (1997).
5.Macounova, K., Cabrera, C. R., Holl, M. R. & Yager, P. Generation of natural pH gradients in microfluidic channels for use in isoelectric focusing. Analytical Chemistry 72, 3745-3751 (2000).
6.Hadd, A. G., Raymond, D. E., Halliwell, J. W., Jacobson, S. C. & Ramsey, J. M. Microchip device for performing enzyme assays. Analytical Chemistry 69, 3407-3412 (1997).
7.Duffy, D. C., Gillis, H. L., Lin, J., Sheppard, N. F. & Kellogg, G. J. Microfabricated centrifugal microfluidic systems: Characterization and multiple enzymatic assays. Analytical Chemistry 71, 4669-4678 (1999).
8.Hadd, A. G., Jacobson, S. C. & Ramsey, J. M. Microfluidic assays of acetylcholinesterase inhibitors. Analytical Chemistry 71, 5206-5212 (1999).
9.Kameoka, J., Craighead, H. G., Zhang, H. W. & Henion, J. A polymeric microfluidic chip for CE/MS determination of small molecules. Analytical Chemistry 73, 1935-1941 (2001).
10.Xu, J., Lee, C. S. & Locascio, L. E. Isoelectric focusing of green fluorescence proteins in plastic microfluid channels. Abstracts of Papers of the American Chemical Society 219, 9-ANYL (2000).
11.Macounova, K., Cabrera, C. R. & Yager, P. Concentration and separation of proteins in microfluidic channels on the basis of transverse IEF. Analytical Chemistry 73, 1627-1633 (2001).
12.Hatch, A. et al. A rapid diffusion immunoassay in a T-sensor. Nature Biotechnology 19, 461-465 (2001).
13.Eteshola, E. & Leckband, D. Development and characterization of an ELISA assay in PDMS microfluidic channels. Sensors and Actuators B-Chemical 72, 129-133 (2001).
14.Cheng, S. B. et al. Development of a multichannel microfluidic analysis system employing affinity capillary electrophoresis for immunoassay. Analytical Chemistry 73, 1472-1479 (2001).
15.Yang, T. L., Jung, S. Y., Mao, H. B. & Cremer, P. S. Fabrication of phospholipid bilayer-coated microchannels for on-chip immunoassays. Analytical Chemistry 73, 165-169 (2001).
16.Sohn, L. L. et al. Capacitance cytometry: Measuring biological cells one by one. Proceedings of the National Academy of Sciences of the United States of America 97, 10687-10690 (2000).
17.Figeys, D., Gygi, S. P., McKinnon, G. & Aebersold, R. An integrated microfluidics tandem mass spectrometry system for automated protein analysis. Analytical Chemistry 70, 3728-3734 (1998).
18.Jiang, Y., Wang, P. C., Locascio, L. E. & Lee, C. S. Integrated plastic microftuidic devices with ESI-MS for drug screening and residue analysis. Analytical Chemistry 73, 2048-2053 (2001).
19.Gao, J., Xu, J. D., Locascio, L. E. & Lee, C. S. Integrated microfluidic system enabling protein digestion, peptide separation, and protein identification. Analytical Chemistry 73, 2648-2655 (2001).
20.Belgrader, P., Okuzumi, M., Pourahmadi, F., Borkholder, D. A. & Northrup, M. A. A microfluidic cartridge to prepare spores for PCR analysis. Biosensors & Bioelectronics 14, 849-852 (2000).
21.Khandurina, J. et al. Integrated system for rapid PCR-based DNA analysis in microfluidic devices. Analytical Chemistry 72, 2995-3000 (2000).
22.Lagally, E. T., Medintz, I. & Mathies, R. A. Single-molecule DNA amplification and analysis in an integrated microfluidic device. Analytical Chemistry 73, 565-570 (2001).
23.Buchholz, B. A. et al. Microchannel DNA sequencing matrices with a thermally controlled "viscosity switch". Analytical Chemistry 73, 157-164 (2001).
24.Fan, Z. H. et al. Dynamic DNA hybridization on a chip using paramagnetic beads. Analytical Chemistry 71, 4851-4859 (1999).
25.Koutny, L. et al. Eight hundred base sequencing in a microfabricated electrophoretic device. Analytical Chemistry 72, 3388-3391 (2000).
26.Lee, G. B., Chen, S. H., Huang, G. R., Sung, W. C. & Lin, Y. H. Microfabricated plastic chips by hot embossing methods and their applications for DNA separation and detection. Sensors and Actuators B-Chemical 75, 142-148 (2001).
27.Glasgow, I. K. et al. Handling individual mammalian embryos using microfluidics. Ieee Transactions On Biomedical Engineering 48, 570-578 (2001).
28.Yang, J., Huang, Y., Wang, X. B., Becker, F. F. & Gascoyne, P. R. C. Cell separation on microfabricated electrodes using dielectrophoretic/gravitational field flow fractionation. Analytical Chemistry 71, 911-918 (1999).
29.Chiu, D. T. et al. Patterned deposition of cells and proteins onto surfaces by using three-dimensional microfluidic systems. Proceedings of the National Academy of Sciences of the United States of America 97, 2408-2413 (2000).
30.Folch, A., Jo, B. H., Hurtado, O., Beebe, D. J. & Toner, M. Microfabricated elastomeric stencils for micropatterning cell cultures. Journal of Biomedical Materials Research 52, 346-353 (2000).
31.Dertinger, S. K. W., Chiu, D. T., Jeon, N. L. & Whitesides, G. M. Generation of gradients having complex shapes using microfluidic networks. Analytical Chemistry 73, 1240-1246 (2001).
32.Jeon, N. L. et al. Generation of solution and surface gradients using microfluidic systems. Langmuir 16, 8311-8316 (2000).
33.Weigl, B. H. & Yager, P. Microfluidics - Microfluidic diffusion-based separation and detection. Science 283, 346-347 (1999).
34.Cunningham, D. D. Fluidics and sample handling in clinical chemical analysis. Analytica Chimica Acta 429, 1-18 (2001).